Lipidomics studies on macrophages - bone marrow-derived macrophages treated with Kdo2-Lipid A and ATP
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Averages of biological replicates - (FA) Fatty acids [cells]
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: View time course graphs for averages of biological replicates| Details | Time course graph (ave of biol. reps) | LM_ID | Name | Mass (neutral) |
Kdo+ATP ATP Kdo Ctrl 0 hrs |
Kdo+ATP ATP Kdo Ctrl 15 min |
Kdo+ATP ATP Kdo Ctrl 30 min |
Kdo+ATP ATP Kdo Ctrl 1 hr |
Kdo+ATP ATP Kdo Ctrl 2 hrs |
Kdo+ATP ATP Kdo Ctrl 4 hrs |
Kdo+ATP ATP Kdo Ctrl 8 hrs |
Kdo+ATP ATP Kdo Ctrl 20 hrs | Units . . . . |
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Legend:
 :Ctrl
 :Kdo2-lipid A
 :ATP
 :ATP+Kdo2-lipid A
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LMFA01030283 | 10Z-heptadecenoic acid | 268.2 (ions) | - - - 0.546 |
1.15 1.07 0.922 0.512 |
0.936 0.744 0.744 0.61 |
0.842 0.785 0.545 0.478 |
0.977 0.738 0.767 0.707 |
0.828 0.789 0.667 0.569 |
1.14 0.749 0.731 0.555 |
1.59 0.823 0.789 0.552 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030159 | 11,14,17-eicosatrienoic acid | 306.3 (ions) | - - - 0.997 |
2.53 1.65 1.11 0.97 |
2.71 1.5 1.08 0.958 |
1.97 1.62 0.979 0.864 |
2.04 1.08 1.06 1.1 |
1.7 1.08 1.06 0.796 |
2.04 1.48 0.852 0.912 |
2.11 1.11 0.929 1.21 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030130 | 11,14-eicosadienoic acid | 308.3 (ions) | - - - 0.163 |
0.205 0.161 0.118 0.136 |
0.171 0.144 0.124 0.131 |
0.148 0.151 0.094 0.102 |
0.177 0.111 0.092 0.121 |
0.168 0.12 0.098 0.099 |
0.209 0.131 0.105 0.131 |
0.228 0.137 0.162 0.157 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030408 | 13,16,19-docosatrienoic acid | 334.3 (ions) | - - - 0.005 |
0.002 0.014 0.023 0.018 |
0.025 0.015 0.01 0.011 |
0.007 0.002 0.001 0.008 |
0.011 0.001 0.006 0.023 |
0.013 0.014 0.004 0.015 |
0.003 0.007 0.004 0.009 |
0.016 0.01 0.006 0.02 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030132 | 13,16-docosadienoic acid | 336.3 (ions) | - - - 0.01 |
0.017 0.008 0.008 0.011 |
0.015 0.007 0.009 0.014 |
0.013 0.012 0.01 0.008 |
0.013 0.008 0.011 0.008 |
0.01 0.004 0.01 0.005 |
0.012 0.008 0.006 0.009 |
0.022 0.008 0.007 0.012 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030759 | 5,8,11,14,17-eicosapentaenoic acid | 302.2 (ions) | - - - 2.19 |
3.02 2.58 1.79 1.91 |
2.95 2.13 1.78 1.87 |
2.44 2.11 1.58 1.54 |
2.79 1.83 1.67 2.02 |
2.21 1.83 1.85 1.58 |
2.81 1.74 2.28 1.79 |
3.16 2.17 2.63 1.93 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030157 | 5,8,11-eicosatrienoic acid | 306.3 (ions) | - - - 0.016 |
0.025 0.016 0.015 0.013 |
0.016 0.011 0.009 0.018 |
0.005 0.008 0.014 0.025 |
0.01 0.014 0.012 0.025 |
0.014 0.009 0.013 0.01 |
0.037 0.012 0.02 0.023 |
0.071 0.042 0.062 0.03 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030184 | 7,10,13,16,19-docosapentaenoic acid | 330.3 (ions) | - - - 1.03 |
2.83 2.09 1.28 1.24 |
2.73 1.75 1.38 1.36 |
2.37 1.79 1.38 1.01 |
2.31 1.46 1.28 1.41 |
1.87 1.35 1.4 1.46 |
2.08 1.07 1.34 1.63 |
3.38 1.71 2.26 1.68 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01030178 | 7Z,10Z,13Z,16Z-docosatetraenoic acid | 332.3 (ions) | - - - 0.363 |
1.03 0.666 0.476 0.423 |
0.99 0.6 0.535 0.488 |
0.913 0.765 0.445 0.407 |
0.921 0.507 0.506 0.579 |
0.779 0.44 0.491 0.481 |
0.792 0.317 0.468 0.575 |
1.11 0.566 0.733 0.672 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030056 | 9Z-palmitoleic acid | 254.2 (ions) | - - - 1.74 |
3.07 2.31 1.91 1.49 |
2.97 2.1 1.78 1.51 |
2.4 2.08 1.78 1.65 |
2.57 1.69 1.9 2 |
1.97 1.57 1.67 1.5 |
3.02 1.98 1.77 1.91 |
2.89 1.96 2.42 1.76 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030152 | alpha-linolenic acid | 278.2 (ions) | - - - 0.092 |
0.103 0.082 0.075 0.069 |
0.107 0.062 0.066 0.062 |
0.074 0.064 0.058 0.068 |
0.082 0.046 0.063 0.051 |
0.07 0.057 0.048 0.042 |
0.094 0.061 0.057 0.038 |
0.073 0.037 0.031 0.031 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01010020 | Arachidic acid | 312.3 (ions) | - - - 2.17 |
2.39 1.85 2.1 1.65 |
2.31 1.5 1.64 1.57 |
2 1.41 1.36 1.55 |
2.56 1.71 1.93 2.19 |
2.38 1.67 1.81 1.54 |
2.15 1.81 1.97 2.02 |
2.44 1.43 1.73 1.32 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030001 | Arachidonic acid | 304.2 (ions) | - - - 7.43 |
18.4 12.1 7.84 7.87 |
18.8 11.4 8.42 7.68 |
14.1 12 7.32 6.62 |
13.9 8.7 7.47 7.59 |
10.3 7.42 8.16 6.96 |
12.3 6.73 8.34 7.55 |
14.4 8.57 9.89 9.01 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01010022 | Behenic acid | 340.3 (ions) | - - - 0.39 |
0.618 0.39 0.406 0.343 |
0.686 0.291 0.408 0.307 |
0.289 0.218 0.367 0.402 |
0.422 0.387 0.319 0.472 |
0.433 0.353 0.435 0.299 |
0.372 0.287 0.395 0.456 |
0.453 0.245 0.241 0.264 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030158 | bishomo-gamma-linolenic acid | 306.3 (ions) | - - - 0.468 |
1.09 0.756 0.594 0.497 |
1 0.608 0.578 0.475 |
0.868 0.759 0.536 0.444 |
0.945 0.575 0.561 0.564 |
0.73 0.496 0.581 0.433 |
1.06 0.565 0.725 0.524 |
1.72 0.802 1.1 0.615 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01010026 | Cerotic acid | 396.4 (ions) | - - - - |
- - - - |
- - - - |
- - - - |
- - - - |
- - - - |
- - - - |
- - - - |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030089 | cis-erucic acid | 338.3 (ions) | - - - 0.111 |
0.136 0.112 0.104 0.116 |
0.125 0.079 0.106 0.119 |
0.128 0.113 0.063 0.094 |
0.158 0.065 0.083 0.103 |
0.152 0.083 0.063 0.097 |
0.113 0.079 0.087 0.121 |
0.235 0.112 0.106 0.09 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01030092 | cis-selacholeic acid | 366.3 (ions) | - - - 0.157 |
0.088 0.091 0.114 0.076 |
0.166 0.109 0.101 0.077 |
0.136 0.078 0.113 0.081 |
0.169 0.088 0.117 0.089 |
0.161 0.108 0.134 0.063 |
0.226 0.121 0.06 0.087 |
0.165 0.098 0.084 0.191 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01030185 | DHA | 328.2 (ions) | - - - 0.905 |
2.02 1.42 1.18 1.02 |
1.94 1.16 1.23 1.11 |
1.67 1.5 1.07 0.988 |
1.76 1.13 1.23 1.29 |
1.66 1.12 1.14 1.09 |
2.01 1.11 1.28 1.27 |
2.73 1.52 2.15 1.58 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01030141 | gamma-linolenic acid | 278.2 (ions) | - - - 0.112 |
0.182 0.178 0.132 0.11 |
0.162 0.108 0.131 0.109 |
0.133 0.13 0.116 0.116 |
0.153 0.11 0.131 0.167 |
0.135 0.106 0.154 0.097 |
0.192 0.151 0.175 0.13 |
0.291 0.191 0.253 0.116 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01010012 | Lauric acid | 200.2 (ions) | - - - 12.8 |
26.2 16.8 17 15.6 |
20.2 16.8 11.7 15.8 |
15.3 14 19.1 10.1 |
18.8 12.8 13.6 15.2 |
17.8 12.3 14.4 16.1 |
11.8 13.4 12.2 20.5 |
17.8 12.4 13.3 12.4 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01010024 | Lignoceric acid | 368.4 (ions) | - - - 0.45 |
0.309 0.257 0.362 0.265 |
0.302 0.16 0.14 0.199 |
0.157 0.143 0.117 0.273 |
0.232 0.18 0.516 0.262 |
0.292 0.232 0.141 0.257 |
0.282 0.34 0.243 0.217 |
0.113 0.233 0.233 0.217 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01030120 | Linoleic acid | 280.2 (ions) | - - - 3.52 |
5.71 4.56 3.98 3.19 |
5.69 3.94 3.91 3.18 |
4.78 3.99 3.57 3.07 |
5.39 3.47 3.67 3.68 |
4.58 3.57 3.65 3.06 |
6.15 4.46 4.24 3.39 |
7.19 4.53 4.18 3.62 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01010017 | Margaric acid | 270.3 (ions) | - - - 2.72 |
3.41 2.49 2.29 2.07 |
3.34 2.13 2.05 2.3 |
2.94 2.32 2.06 2.17 |
3.5 2.65 2.49 2.07 |
3.11 2.56 2.39 2.04 |
2.88 2.5 2.57 2.34 |
3.46 2.33 2.37 1.68 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01010014 | Myristic acid | 228.2 (ions) | - - - 12.7 |
26.1 19.6 17 16.5 |
22.5 14.9 16 17.1 |
18.8 15.2 14.3 14 |
21.4 16 16.4 19.1 |
18 15.3 15.7 19.5 |
19.2 18.3 16.9 21 |
18 13.9 16.2 13.2 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01030002 | Oleic acid | 282.3 (ions) | - - - 45.6 |
88.7 73.1 54 47.6 |
79 55.7 53.3 48.1 |
69.7 61.7 45.6 42.7 |
77.6 51.5 49.5 48.7 |
66.3 50.8 44.7 42.6 |
90.3 67.3 52.4 51.6 |
106 62 61 54.4 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01010001 | Palmitic acid | 256.2 (ions) | - - - 106 |
153 121 120 86.8 |
139 103 95.4 96 |
126 105 88.2 74.4 |
141 107 106 108 |
119 103 95.8 99 |
129 116 118 116 |
132 91.7 109 99.6 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01010015 | Pentadecanoic acid | 242.2 (ions) | - - - 3.05 |
5.33 4.14 4.38 3.4 |
4.59 3.57 3.5 3.19 |
4.24 2.95 2.84 2.95 |
5 4.04 4.13 3.34 |
4.29 3.37 3.63 3.27 |
4.22 3.71 3.8 3.84 |
4.15 3.02 2.89 3 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01010018 | Stearic acid | 284.3 (ions) | - - - 75.6 |
131 98 99.9 73.4 |
128 84.8 87 85 |
113 94.3 84.4 70.6 |
132 99.9 101 95.9 |
122 101 92.9 87.3 |
135 106 99.4 113 |
141 90.4 99.3 89.8 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
|
LMFA01030357 | Stearidonic acid | 276.2 (ions) | - - - 0.018 |
0.007 0.007 0.006 0.005 |
0.002 0.003 0.007 0.002 |
0.004 0.003 0.004 0.018 |
0.008 0.001 0.004 0.021 |
0.01 0.002 0.003 0.005 |
0.003 0.002 0.003 0.002 |
0.011 0.001 - 0 |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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LMFA01010023 | Tricosanoic acid | 354.3 (ions) | - - - - |
- - - - |
- - - - |
- - - - |
- - - - |
- - - - |
- - - - |
- - - - |
pmol/ug DNA |
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Legend:
:Ctrl
:Kdo2-lipid A
:ATP
:ATP+Kdo2-lipid A
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- | DNA | - | - - - 10.6 |
9.6 9.86 10.1 9.53 |
11 10.9 9.3 11.2 |
7.65 9.19 9.94 9.52 |
7.47 7.96 8.5 11.1 |
6.3 9.33 12.5 11.5 |
8.41 9.99 8.94 11.4 |
7.71 10.9 12.7 11.9 |
ug/sample |