LMPD Database

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LMP000303

UniProt Annotations

Entry Information
Gene Nameinositol polyphosphate-5-phosphatase, 75kDa
Protein EntryB3KMW4_HUMAN
UniProt IDP32019
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=4; Name=1; IsoId=P32019-1; Sequence=Displayed; Note=No experimental confirmation available.; Name=2; IsoId=P32019-2; Sequence=VSP_012820; Name=3; IsoId=P32019-3; Sequence=VSP_012821; Name=4; IsoId=P32019-4; Sequence=VSP_013902, VSP_013903; Note=No experimental confirmation available.;
Catalytic Activity1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
DomainThe ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature, all three interactions relie on F&H motifs, an approximately 12-13 amino-acid sequence centered around Phe and His residues essential for binding (By similarity).
FunctionHydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.
PtmIsoprenylation at Cys-990 may be required for localization at the membrane.
PtmMay be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form.
Sequence CautionSequence=AAA79207.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ;
SimilarityBelongs to the inositol 1,4,5-trisphosphate 5- phosphatase type II family.
SimilarityContains 1 PH domain.
SimilarityContains 1 Rho-GAP domain. {ECO
Subcellular LocationCytoplasm, cytosol. Endoplasmic reticulum- Golgi intermediate compartment. Early endosome membrane. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane . Golgi apparatus.
SubunitInteracts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating the phosphatase activity. {ECO
Tissue SpecificityPlatelets.