LIPID MAPS -- LIPID Metabolites And Pathways Strategy

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Publications

Chen, Y., Allegood, J. C., Liu, Y., Wang, E., Cox, T. M., Merrill, A. H., Jr., and Sullards, M. C., (2008) Imaging MALDI mass spectrometry using an oscillating capillary nebulizer matrix coating system and its application to analysis of lipids in brain from a mouse model of Tay-Sachs/Sandhoff disease, Anal Chem, in press.

Gibbons, H. S., Reynolds, C. M., Guan, Z., and Raetz, C. R., (2008) An inner membrane dioxygenase that generates the 2-hydroxymyristate moiety of Salmonella lipid A, Biochemistry, Epub ahead of publication.
PubMed ID: 18254598

Harkewicz, R., Hartvigsen, K., Almazan, F., Dennis, E. A., Witztum, J. L., and Miller, Y. I., (2008) Cholesteryl ester hydroperoxides are biologically active components of minimally oxidized LDL, J Biol Chem, Epub ahead of publication.
JBC article

Haynes, C. A., Allegood, J. C., Sims, K., Wang, E., Sullards, M. C., and Merrill, A. H., Jr., (2008) Quantitation of fatty acyl-coenzyme A molecular species in mammalian cell extracts by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI MS/MS), J Lipid Res, Epub ahead of publication.
J Lipid Res article

Hutchins, P., and Murphy, R. C., (2008) Separation of cellular non–polar neutral lipids by normal phase chromatography and analysis by electrospray ionization mass spectrometry, J Lipid Res, Epub ahead of publication.
J Lipid Res article

Sasaki, H., and White, S. H., (2008) Aggregation behavior of an ultra-pure lipopolysaccharide (LPS) that stimulates TLR-4 receptors, Biophys J, in press.

Schaaf, G., Ortlund, E. A., Tyeryar, K. R., Mousley, C. J., Ile, K. E., Garrett, T. A., Ren, J., Woolls, M. J., Raetz, C. R., Redinbo, M. R., and Bankaitis, V. A., (2008) Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily, Mol Cell, 29, 191-206.
PubMed ID: 18243114

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LIPID MAPS is funded by an National Institute of General Medical Sciences (NIGMS) Glue Grant

LIPID MAPS Highlights

Guided Tour of
the LIPID MAPS Website

Methods in Enzymology Chapters

Methods in Enzymology, Academic Press, Vol 432

New! Download all LIPID MAPS Methods in Enzymology Vol 432 Chapters as a single PDF.

Deems, R. A., Buczynski, M. W., Bowers-Gentry, R. C., Harkewicz, R., and Dennis, E. A. (2007) Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry. in Meth. Enzymol., Academic Press, 432, 59-82.

Fahy, E., D. Cotter, R. Byrnes, M. Sud, A. Maer, J. Li, D. Nadeau, Y. Zhou, and S. Subramaniam. Bioinformatics for Lipidomics (2007). Meth. Enzymol. (432), 247-273.

Garrett, T. A., Guan, Z., and Raetz, C. R. (2007) Analysis of ubiquinones, dolichols and dolichol diphosphate-oligosaccharides by liquid chromatography-electrospray ionization mass spectrometry. in Meth. Enzymol., Academic Press, 432, 117-142.

Ivanova, P., Milne, S., Byrne, M. O., Xiang, Y., and Brown, H. A. (2007) Glycerophospholipid identification and quantitation by electrospray mass spectrometry. in Meth. Enzymol., Academic Press, 432, 21-57.

Krank, J., Murphy, R. C., Barkley, R. M., Duchoslav, E., and McAnoy, A. (2007) Qualitative analysis and quantitative assessment of changes in neutral glycerol lipid molecular species within cells. in Meth. Enzymol., Academic Press, 432, 1-20.

McDonald, J. G., Thompson, B., McCrum, E. C., and Russell, D. W. (2007) Extraction and analysis of sterols in biological matrices by high-performance liquid chromatography electrospray ionization mass spectrometry. in Meth. Enzymol., Academic Press, 432, 143-168.

Moore, J. D., Caufield, W. V., and Shaw, W. (2007) Quantitation and standardization of lipid internal standards for mass spectroscopy. in Meth. Enzymol., Academic Press, 432, 351-367.

Schmelzer, K., Fahy, E., Deems, R., Subramaniam, S., and Dennis, E. A. (2007) The Lipid Maps Initiative in Lipidomics. in Meth. Enzymol., Academic Press, 432, 169-181.

Sullards, M. C., Allegood, J. C., Kelly, S., Wang, E., Haynes, C. A., Park, H., Chen, Y., Shaner, R., and Merrill, A. H., Jr. (2007) Structure-specific, quantitative methods for analysis of sphingolipids by liquid-chromatography tandem mass spectrometry: "Inside-out" sphingolipidomics. in Meth. Enzymol., Academic Press, 432, 83-115.

Methods in Enzymology, Academic Press, Vol 433

Garrett, T. A., Kordestani, R., and Raetz, C. R. (2007) Quantification of cardiolipin by liquid chromatography-electrospray ionization mass spectrometry. in Meth. Enzymol., Academic Press, 433, 213-230.

Methods in Enzymology, Academic Press, Vol 434

Brown, H. A., Henage, L. G., Preininger, A. M., Xiang, Y., and Exton, J. H. (2007) Biochemical analysis of phospholipase D. in Meth. Enzymol., Academic Press, 434, 49-87.

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07/28/08 - We have expanded our classification system to include lipids from a variety of non-mammalian sources such as plants, bacteria and marine organisms. We have also added expandable/collapsable menus to facilitate browsing. The LIPID MAPS structure database now contains a total of 10,789 entries, including 2688 Fatty acyls (FA), 3009 Glycerolipids (GL), 1971 Glycerphospholipids (GP), 621 Sphingolipids (SP), 1745 Sterol lipids (ST), 609 Prenol lipids (PR), 10 Saccharolipids (SL), and 136 Polyketides (PK).

04/29/08 - Download all LIPID MAPS Methods in Enzymology Vol 432 Chapters as a single PDF.

03/07/08 - A number of new web-based MS prediction tools for analysis of data on glycerophospholipids, glycerolipids, cardiolipins, sphingolipids, fatty acids and cholesteryl esters have been added to the Tools section of the LIPID MAPS website. These tools allow end-users to enter peaklists of precursor ion data and obtain a table of predicted molecular species, based on permutations of commonly occurring side-chains and/or known lipid structures. A standalone Windows version is also available for download.

03/06/08 - LMSD Structure-data file (SDF) updated: Download exports of structures and annotations from the LIPID MAPS Structure Database (LMSD). Annotations include: LM ID, Common Name, Systematic Name, Exact Mass, Formula, Category, Main Class, Subclass, Synonyms, PubChem Substance ID (SID), and Status.

03/06/08 - LIPID MAPS Tools Package Updated: The LIPID MAPS Tools Package contains command-line Perl scripts to generate SD files containing structure and ontological data for various lipid categories. The current version supports structure generation for Fatty acyls, Glycerolipids, Glycerophospholipids, Cardiolipins, Sphingolipids and Sterols.

03/06/08 - LIPID MAPS Discussion Board Publicly accessible discussion forum. Current topics include: Lipids: General, Lipid Classification, Lipid Mass Spectrometry, Lipid Extraction and Purification, Lipid Biochemistry, and Lipid-related Biochemical Pathways, and Research Ideas & Applications Generated from LIPID MAPS Data.

02/13/08 - Guided Tour of the LIPID MAPS Website: A walk through the LIPID MAPS website in which we highlight key features of the website; introduce some soon to be released features, including lipid pathways and additional data from lipidomics studies on macrophages; and discuss some of our in-house software for data analysis.

02/13/08 - Lipidomics Studies on Macrophages: RAW264.7 cells: Browse, search, and download data from experiments conducted on RAW264.7 cells treated with Kdo₂-Lipid A. Measurements were taken at 0, 0.5, 1, 2, 4, 8, 12, and 24hrs for both Kdo2-Lipid A and control.

02/06/08 - Gene array data on macrophages: Browse and search data from experiments conducted on RAW264.7 cells treated with Kdo₂-Lipid A and Kdo₂-Lipid A + compactin plus experiments on thioglycolate-elicited and bone-marrow derived primary macrophages treated with Kdo₂-Lipid A, including data from foam cell studies with LDLR(-/-) and wild-type mice. Query options include filtering by lipid category, gene symbol, KEGG pathway, fold-change, and more.

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