Microarray analysis of RAW 264.7 cells treated with Kdo2-Lipid A

Introduction: Experimental design

Microarray analysis was used to find gene expression response in RAW264.7 cells over time. Two groups of control and two groups of RAW 264.7 cells were harvested 30m,60m,90m,2h,4h,6h,8h,12h,18h, and 24h after treatment with 100ng/ml Kdo2-Lipid A. At each time point 2 Agilent arrays were hybridized, one with Kdo2-Lipid A on the green channel (control on RED) and the other with Kdo2-Lipid A on the red (control on the GREEN) i.e. dye-swapped. Cells were grown in 0.5% fetal calf serum and handled as described in our Lipid Induction Protocol.