Contact | Discussion | News | Publications | Site Map
	LIPID Metabolites And Pathways Strategy

About Lipid Classification Standards Experimental Data Databases Pathways Tools Protocols Home

Analysis of RAW 264.7 Cells

Microarray Experiments

For the following experiments, microarray analysis was used to measure gene expression response in RAW264.7 cells over time. In each experiment, RAW 264.7 cells were grown in 10% fetal calf serum and hybridized with Agilent Whole Mouse Genome DNA microarrays.

• Kdo2-Lipid A

  Cells were harvested at each of ten time points — 0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, 12.0, 18.0, and 24 hours — after treatment with 100ng/ml Kdo2-Lipid A. The experiment was performed three times, for three biological replicates. At each time point an Agilent array was hybridized, with Kdo2-Lipid A on the green channel and the control on the red channel. One biological replicate was also run with Kdo2-Lipid A on the red channel and the control on the green channel (i.e. dye-swapped). We evaluated these data and found no dye effects (data not shown).