Lipidomics studies on macrophages
Thioglycolate-elicited Macrophage Time Course Study with Kdo2-Lipid A and Compactin
Compactin is a potent competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase, the rate-limiting enzyme in cholesterol biosynthesis. Experiments were conducted with peritoneal macrophages, obtained from thioglycolate-stimulated mice, as per protocols PP0000001401, PP0000001502 and PP0000001801.
For the 8 time point study, cells were treated for 0, 0.5, 1, 2, 4, 8, 12, or 24 hours with Kdo2-Lipid A. Untreated cells were used as a zero timepoint. Three separate experiments (biological replicates) were carried out, and a single MS measurement was performed on each sample. Measurements were taken at each timepoint for: (i) Kdo2-Lipid A + compactin, (ii) Kdo2-Lipid A, (iii) compactin, and (iv) control.
BrowseAverages of biological replicates, by analyte category
|
DownloadDownload timecourse data as a comma-delimited (CSV) or tab-delimited (TSV) file |
References
Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4.
Raetz C, Garrett T, Reynolds C, Shaw W, Moore J, Smith JD, Ribeiro A, Murphy R, Ulevitch R, Fearns C, Reichart D, Glass C, Benner C, Subramaniam S, Harkewicz R, Bowers-Gentry R, Buczynski M, Cooper J, Deems R and Dennis E.
Journal of Lipid Research 47, 1097-1111 (2006)
