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LIPID Metabolites And Pathways Strategy |
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Macrophages were isolated from bone marrow progenitor cells obtained from the long bones of mice, as per protocols PP0000003500 and PP0000001801 (pdf). Cells were treated for 6 or 24 hours with Kdo2-Lipid A. Untreated cells were used as a zero timepoint. Three separate experiments (biological replicates) were carried out, and a single MS measurement was performed on each sample.